Preparation of 1 N Sulfuric Acid (H2SO4):
- Take a 100 mL of purified water or water in 1000 mL volumetric flask. Add 27.0 ml of sulfuric acid carefully.
- Add approx. 700 mL water. Allow Solution to cool down to Room Temperature.
- Make up the volume upto 1000 mL with purified water.
Calculation:
Molecular weight of Sulfuric Acid (H2SO4)= 98.079 g/mol
Related: Preparation and Standardization of 0.1N Hydrochloric Acid (HCL)
Standardization 1 N Sulfuric Acid (H2SO4) using Tromethamine (THAM) or Tris, or trisaminomethane:
- Accurately weigh about 5.0 g of Tromethamine, dried according to the label of manufacturer or dried at 105° for 3 h.
- Dissolve THAM in 50 mL of water and add 2 drops of bromocresol green as a indicator. Titrate with 1 N sulfuric acid to a pale yellow endpoint.
- Repeat the procedure for three time and record the observation.
Calculation:
- Each 121.14 mg of tromethamine is equivalent to 1 mL of 1 N sulfuric acid.
Related: Preparation and standardization of 0.1 N Sodium Hydroxide (NaOH)
Preparation of HPLC Mobile Phase
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