Autoclave Sterilization Cycle or Autoclaving Process

Autoclave Sterilization Cycle or Autoclaving Process

  • Autoclave Sterilization Cycle : The autoclave is frequently used to sterilize various pieces of equipment and media in practical labs, businesses, and healthcare facilities.
  • Items are placed within an autoclave chamber under controlled temperature, pressure, and steam conditions for a predetermined amount of time, either wrapped or placed directly over the holding plate.
  • An autoclave functions basically similarly to a pressure cooker, which we frequently use to boil or cook food.
  • It could close the door or lid of an autoclave to create a sealed chamber similar to one in a pressure cooker.
  • Afterward, depending on the type of autoclave, air displacement systems like steam, steam flushes, and vacuum systems remove the air from the pressure vessel.
  • After then, by raising the autoclave’s temperature and killing power- ability, the pressure inside the device enables successful sterilization at the specified time.
  • After the media and equipment have been sterilized, release the steam.
  • There are three stages or Phases in the autoclave sterilization cycle as a result, depending on how an autoclave operates:
  1. Purge Phase
  2. Exposure Phase
  3. Exhaust Phase
Autoclave Sterilization Cycle or Autoclaving Process

Autoclave Sterilization Cycle Process:

A. Purge Phase (Conditioning Phase):

  • Additionally, it describes the as “Conditioning Phase”, which entails or required for displacing or removing air from the pressure vessel that can obstruct the sterilization procedure.
  • To remove air from the chamber, different autoclaves employ various techniques.
    • Steam is used to displace the air in a “Gravity Displacement Autoclave”.
    • It employs pressure pulses and steam flushes for its “Positive Pressure Displacement Autoclave”.
    • Negative pressure (vacuum) displacement type, in which all the air is removed using a vacuum pump.

B. Exposure Phase or Sterilization Phase :

  • In order to continuously deliver steam into the pressure vessel through a heating element during the sterilizing phase, it must be closed the autoclave’s exhaust valve.
  • It causes the temperature and pressure to quickly rise to the appropriate setpoint.
  • Then, depending on the kind and load of the content, the chamber’s contents undergo the exposure phase under sterilization temperature exposure for the necessary amount of time.

C. Exhaust Phase:

  • The final phase includes the release of an exhaust valve to release steam out of the autoclave chamber or depressurize it.
  • As the steam entirely evaporates, and take out the sterilized material.

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