Difference Between Thin Layer and Paper Chromatography

TLC vs Paper Chromatography

Sr. No.PointThin-layer chromatography (TLC)Paper Chromatography
1PrincipleThin-layer chromatography (TLC) is based on the adsorption principle.This chromatography is based on the partition principle.
2Mobile PhaseMobile Phase can be liquid of gas.The liquid will be the Mobile phase.
3Stationary PhaseGlass plates or plastic sheets coated with a thin layer of a finely ground adsorbent, such as silica gel or aluminaPaper sheets or strips as the adsorbent material
4In Organic Ion AnalysisNot properly suitableInorganic ions can also gladly be separated on paper chromatography than compare to  thin-layer chromatography (TLC).  
5For color-forming agents & strong solventsThin-layer chromatography has a diverse benefit over paper chromatography in that the TLC plate or sheet is able to withstand color-forming agents & strong solventsNot properly suitable
6TypeThin Layer Chromatography1. Descending paper chromatography.

2. Ascending paper chromatography.

3. Radial paper chromatography.

4. Descending-Ascending paper chromatography.

5. Two dimensional paper chromatography.
7AdvantagesCorrosive reagents can be detectable.
Easy in use for UV detection.
Choice of Spray Reagents
Automation can be done in TLC over Paper Chromatography
Development Chamber Design can be easy than paper chromatography.
The separation method is sharper.
The sensitivity and spot of sample diffusion is very low.
It is a simple, low cost and hassle-free operations technique.
Less preparation time & analysis time required
The separation method is less sharp.
The sensitivity and spot of sample diffusion is high than TLC.
TLC vs Paper Chromatography

Difference Between TLC and Paper Chromatography
Difference between paper and thin layer chromatography


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