Here are some basic questions and answers about different types of chromatography:
Types of Chromatography- Que & Ans
1. What is gas chromatography (GC)?
- Gas chromatography is a technique used to separate and analyze volatile compounds. It involves the use of a gas as the mobile phase and a stationary phase packed in a column.
2. What is liquid chromatography (LC)?
- Liquid chromatography is a broad category of chromatographic techniques that use a liquid mobile phase to separate and analyze components in a sample.
3. What is high-performance liquid chromatography (HPLC)?
- High-performance liquid chromatography (HPLC) is a widely used form of liquid chromatography that utilizes high-pressure pumps to pass the liquid mobile phase through a column packed with a stationary phase.
4. What is ion chromatography (IC)?
- Ion chromatography is a type of liquid chromatography used to separate and analyze ions in a sample. It employs ion-exchange resins as the stationary phase.
5. What is affinity chromatography?
- Affinity chromatography is a technique that utilizes specific biological interactions, such as antigen-antibody or receptor-ligand binding, to separate and purify target compounds from a mixture.
6. What is thin-layer chromatography (TLC)?
- Thin-layer chromatography involves the separation of compounds on a thin layer of adsorbent material coated on a solid support. It is commonly used for qualitative analysis and identification of compounds.
7. What is size-exclusion chromatography (SEC)?
- Size-exclusion chromatography is a technique used to separate molecules based on their size and molecular weight. It employs a porous stationary phase that allows smaller molecules to penetrate and take longer to elute.
8. What is paper chromatography?
- Paper chromatography is a type of chromatography where a paper strip acts as the stationary phase. It is often used for the separation of pigments and dyes.
9. What is column chromatography?
- Column chromatography is a technique where the stationary phase is packed into a column and the mobile phase is passed through it. It is used for purification and separation of compounds.
10. What is chiral chromatography?
- Chiral chromatography is a type of chromatography used to separate enantiomers, which are mirror-image isomers of a molecule that are not superimposable.
11. What is supercritical fluid chromatography (SFC)?
- Supercritical fluid chromatography is a technique that utilizes supercritical fluids, such as carbon dioxide, as the mobile phase. It is particularly useful for the separation of nonvolatile and thermally labile compounds.
12. What is reversed-phase chromatography?
- Reversed-phase chromatography is a type of liquid chromatography where the stationary phase is nonpolar and the mobile phase is polar. It is commonly used for the separation of hydrophobic compounds.
13. What is hydrophilic interaction chromatography (HILIC)?
- Hydrophilic interaction chromatography is a type of liquid chromatography where the stationary phase is polar and the mobile phase is less polar. It is used for the separation of polar compounds.
14. What is preparative chromatography?
- Preparative chromatography is a technique used to isolate and purify larger quantities of compounds for further analysis or use. It typically involves larger-scale columns and higher sample loads.
15. What is chromatography used for in analytical chemistry?
- Chromatography is used in analytical chemistry to separate, identify, and quantify components in a sample. It is widely employed in various fields, including pharmaceuticals, environmental analysis, forensics, and food and beverage testing.
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