What is UPLC (Ultra Performance Liquid Chromatography)?
UPLC Definition: UPLC’s full name is Ultra Performance Liquid Chromatography. Because of its ability to increase sample throughput, chromatographic efficiency, sensitivity, and reduce run time, UPLC chromatography has become the modern standard HPLC platform.
Pressure: UPLC system can operate at pressure up to 15,000 psi.
Credit@ Waters Corporation, USA
What is the Difference Between UPLC and HPLC?
The liquid chromatography techniques HPLC and UPLC are both used to separate the different components contained in mixtures.
UPLC, on the other hand, uses higher pressures (15,000 psi) and allows for smaller particle sizes in the columns, whereas HPLC uses lower pressures (max 6000 psi).
Particle sizes: UPLC has a stronger sensitivity as well as the ability to handle higher pressure levels. As a result, it is suitable for low-component analysis since it allows for smaller particle sizes within columns.
UPLC improves analyte resolution and sensitivity while also reducing solvent usage and reducing run times.
UPLC is a common laboratory technique that decreases the cost of designing and validating a method while increasing the efficiency of analysis.
The work of Russian botanist Mikhail S. Tswett defined liquid chromatography (LC) in the early 1900s. In 1967, Waters Associates (now Waters Corporation), business that provides analytic tools and systems to scientists, developed the first commercially available HPLC system, the ALC100 HPLC.
Waters Corporation USA, in the year 2004, launched and trademarked Ultra Performance Liquid Chromatography (UPLC).
FPLC full name is Fast protein liquid chromatography (FPLC), is a method of liquid chromatography that is frequently used to analyze or purify mixtures of proteins.
FPLC was developed and promoted in Sweden by Pharmacia in the year 1982, and was originally called fast-performance liquid chromatography (FPLC) to difference it with HPLC (High-performance liquid chromatography).
Although FPLC is typically used to separate proteins, it has a wide range of applications due to the variety of resins and buffers available.