Clean the UV Cabinet from outside and inside with a clean dry cloth.
Switch on the main power supply.
Open the cabinet door by rotating the knob in either direction.
Keep the TLC Plate in U.V. Cabinet.
Close the cabinet door by rotating the knob in either direction.
Turn “ON” the main switch.
Switch on the required UV Light i.e. Short wave (254 nm), Longwave (366 nm).
Visualize the plate through an eyepiece from outside the chamber.
Switch off the UV Light i.e. Short wave (254 nm), Longwave (366 nm).
Turn “OFF” the main switch.
Open the cabinet door and remove the plate from the chamber and simultaneously close the door.
Precaution during the handling of UV Cabinet
Do not look directly with naked eyes towards the light, when using Long and Short waves.
Never work with unprotected hands under UV light, e.g. for marking or adding texts to TLC plates.
Use a soft cloth to wipe off the glass from time to time.
Before starting ensure that the UV Cabinet is clean.
Calibration of UV Lamp
Frequency of Calibration: 6 Months.
Procedure for a short wave (254 nm) calibrationof UV Cabinet
Preparation of Sodium salicylate solution(C7H5NaO3) (Concentration: 0.4 g/L): Accurately weigh and transfer about 100 mg of Sodium salicylate into a 250-mL volumetric flask. Dissolve it insufficient amount of alcohol and dilute to volume with the alcohol.
Spot approximately 5 µL of the above solution of Sodium salicylate on the silica gel 60 F254 TLC plate. The spot shall have a diameter of about 5 mm.
Allow the spot to dry in the air and examine the Sodium salicylate spot on the TLC plate under Short wave (254 nm).
Acceptance Criteria: The lamp shall be capable of revealing the spot on the silica gel 60 F254 TLC plate under a Short wave (254 nm).
Procedure for a Longwave (366 nm) calibration of UV Cabinet
Preparation of Sodium salicylate solution (C7H5NaO3) (Concentration: 2 g/L): Accurately weigh and transfer about 200 mg of Sodium salicylate into a 100 mL volumetric flask. Dissolve it in sufficient amount of alcohol, make up the volume with the alcohol.
Spot approximately 5 µL of the above solution of Sodium salicylate on the silica gel 60 F254 TLC plate. The spot shall have a diameter of about 5 mm.
Allow the spot to dry in air and examine Sodium salicylate spot on the TLC plate under Longwave (366 nm).
Acceptance Criteria: The lamp shall be capable of revealing the spot on the silica gel 60 F254 TLC plate under Longwave (366 nm).